enhancer (New England Biolabs)
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Structured Review
New England Biolabs
enhancer
Enhancer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modules/NEBNext+Ultra+II+Ligation+Module/pm42906542-1219-42-43
Average 97 stars, based on 534 article reviews
Enhancer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modules/NEBNext+Ultra+II+Ligation+Module/pm42906542-1219-42-43
Average 97 stars, based on 534 article reviews
enhancer - by Bioz Stars,
2026-11
97/100 stars
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Formalin-fixed Paraffin-Embedded:Article Title: Chromosome-scale assembly of the African yam bean genome. Article Snippet: .. For each run, this involved repairing and 3’ adenylation of 1 μg of HMW genomic DNA with the NEBNext FFPE DNA Repair Mix and the NEBNext® UltraTM II End Repair/dA-Tailing Article Title: Chromosome-scale assembly of the African yam bean genome Article Snippet: .. For each run, this involved repairing and 3’ adenylation of 1 μg of HMW genomic DNA with the NEBNext FFPE DNA Repair Mix and the NEBNext® UltraTM II End Repair/dA-Tailing Article Title: Genome sequencing and molecular networking analysis of the wild fungus Anthostomella pinea reveal its ability to produce a diverse range of secondary metabolites Article Snippet: For long-read sequencing, the genomic DNA was prepared using the ligation sequencing kit SQK-LSK112 (Oxford Nanopore Technologies, Oxford, United Kingdom) according to the manufacturer’s guidelines. .. Briefly, genomic DNA (1020 ng) was subjected to end repair, 5’ phosphorylation and dA-tailing by NEBNext FFPE DNA Repair and NEBNext Ultra II End prep Purification:Article Title: TET2-dependent differential 5hmC deposition balances adult neural stem cell activation and differentiation Article Snippet: Three μg of genomic DNA was sheared using Covaris S2 and was spiked with two 210-base-pair PCR amplicons (C amplicon and 5hmC amplicon) at a ratio of around 1:20,000. .. DNA was purified with Ampure XP beads (Beckman Coulter) and processed with NEBNext End Repair and dA-Tailing Article Title: OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide. Article Snippet: Unmethylated lambda DNA (Promega, D1521) was spiked into the genomic DNA samples at a ratio of 1:200 to monitor the bisulfite conversion efficiency. .. The samples were then sheared using Covaris S2, purified with Ampure XP beads (Beckman Coulter), processed with NEBNext End Repair and dA-Tailing Article Title: OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide Article Snippet: Unmethylated lambda DNA (Promega, D1521) was spiked into the genomic DNA samples at a ratio of 1:200 to monitor the bisulfite conversion efficiency. .. The samples were then sheared using Covaris S2, purified with Ampure XP beads (Beckman Coulter) and processed with NEBNext End Repair and dA-Tailing Ligation:Article Title: TET2-dependent differential 5hmC deposition balances adult neural stem cell activation and differentiation Article Snippet: Three μg of genomic DNA was sheared using Covaris S2 and was spiked with two 210-base-pair PCR amplicons (C amplicon and 5hmC amplicon) at a ratio of around 1:20,000. .. DNA was purified with Ampure XP beads (Beckman Coulter) and processed with NEBNext End Repair and dA-Tailing Article Title: OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide. Article Snippet: Unmethylated lambda DNA (Promega, D1521) was spiked into the genomic DNA samples at a ratio of 1:200 to monitor the bisulfite conversion efficiency. .. The samples were then sheared using Covaris S2, purified with Ampure XP beads (Beckman Coulter), processed with NEBNext End Repair and dA-Tailing Article Title: Preclinical development of lentiviral vector gene therapy for Diamond-Blackfan anemia syndrome. Article Snippet: 451 Lentiviral insertion sites were determined as described before.21,34 Briefly, 1 μg of genomic DNA 452 was sheared to generate fragments between 200 and 800 bp using a Bioruptor® Pico sonication 453 device (Diagenode, #B01080010). .. DNA underwent end-repair, dA-tailing, and ligation of adaptors 454 with New England BioLabs (NEB) Article Title: OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide Article Snippet: Unmethylated lambda DNA (Promega, D1521) was spiked into the genomic DNA samples at a ratio of 1:200 to monitor the bisulfite conversion efficiency. .. The samples were then sheared using Covaris S2, purified with Ampure XP beads (Beckman Coulter) and processed with NEBNext End Repair and dA-Tailing Methylation:Article Title: TET2-dependent differential 5hmC deposition balances adult neural stem cell activation and differentiation Article Snippet: Three μg of genomic DNA was sheared using Covaris S2 and was spiked with two 210-base-pair PCR amplicons (C amplicon and 5hmC amplicon) at a ratio of around 1:20,000. .. DNA was purified with Ampure XP beads (Beckman Coulter) and processed with NEBNext End Repair and dA-Tailing Article Title: OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide. Article Snippet: Unmethylated lambda DNA (Promega, D1521) was spiked into the genomic DNA samples at a ratio of 1:200 to monitor the bisulfite conversion efficiency. .. The samples were then sheared using Covaris S2, purified with Ampure XP beads (Beckman Coulter), processed with NEBNext End Repair and dA-Tailing Article Title: OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide Article Snippet: Unmethylated lambda DNA (Promega, D1521) was spiked into the genomic DNA samples at a ratio of 1:200 to monitor the bisulfite conversion efficiency. .. The samples were then sheared using Covaris S2, purified with Ampure XP beads (Beckman Coulter) and processed with NEBNext End Repair and dA-Tailing Phospho-proteomics:Article Title: Genome sequencing and molecular networking analysis of the wild fungus Anthostomella pinea reveal its ability to produce a diverse range of secondary metabolites Article Snippet: For long-read sequencing, the genomic DNA was prepared using the ligation sequencing kit SQK-LSK112 (Oxford Nanopore Technologies, Oxford, United Kingdom) according to the manufacturer’s guidelines. .. Briefly, genomic DNA (1020 ng) was subjected to end repair, 5’ phosphorylation and dA-tailing by NEBNext FFPE DNA Repair and NEBNext Ultra II End prep Magnetic Beads:Article Title: Genome sequencing and molecular networking analysis of the wild fungus Anthostomella pinea reveal its ability to produce a diverse range of secondary metabolites Article Snippet: For long-read sequencing, the genomic DNA was prepared using the ligation sequencing kit SQK-LSK112 (Oxford Nanopore Technologies, Oxford, United Kingdom) according to the manufacturer’s guidelines. .. Briefly, genomic DNA (1020 ng) was subjected to end repair, 5’ phosphorylation and dA-tailing by NEBNext FFPE DNA Repair and NEBNext Ultra II End prep Polymerase Chain Reaction:Article Title: The priority of yeast to select among various DNA options to repair genome breaks by homologous recombination. Article Snippet: Background Horizontal gene transfer (HGT) is considered an important mechanism to contribute to the evolution of bacteria, plants, and animals by allowing the movement of genetic material between organisms, in difference to vertical inheritance.. Thereby it can also play a significant role in spreading traits like antibiotic resistance among bacteria and virulence factors between pathogens.. During the HGT, organisms take up free DNA from the environment and incorporate it into their genomes. |